celltrace violet (ctv) dye (Thermo Fisher)

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Thermo Fisher celltrace violet (ctv) dye
Celltrace Violet (Ctv) Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltrace+violet+dye+ctv/celltrace+violet/pmc11883473-118-5-9
Average 90 stars, based on 1 article reviews

celltrace violet (ctv) dye - by Bioz Stars, 2026-09

90/100 stars

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other:

Article Title: Pre-clinical data supporting immunotherapy for HIV using CMV-HIV-specific CAR T cells with CMV vaccine
Article Snippet: CellTraceTM Violet dye (CTV) was purchased from Invitrogen (Carlsbad, CA).

Allele-specific Oligonucleotide:

Article Title: Targeting ZC3H11A elicits immunogenic cancer cell death through augmentation of antigen presentation and interferon response
Article Snippet: .. For DC phagocytosis, ASO-treated cells were stained with CellTrace violet dye (CTV, 5 μg/mL) (Thermo Fisher Scientific) and co-cultured with imDCs for 2 h. The CTV + DCs were considered phagocytic cells and were quantified by flow cytometry after gating for CD11c + (phycoerythrin [PE] anti-mouse CD11c [BioLegend]). .. For DC maturation, imDCs were cocultured with ASO-treated cells for 24 h. The DC maturation markers were analyzed by flow cytometry after staining with the following Abs: PE anti-mouse CD11c (BioLegend), BB515 rat anti-CD11b (BD Biosciences), Brilliant Violet 510TM anti-mouse I-A/I-E (BioLegend), PE/Cy5 anti-mouse CD40 (BioLegend), and APC anti-mouse CD86 (BioLegend), all prepared with a dilution of 1:200.

Staining:

Flow Cytometry:

Article Title: Efferocytosis of SARS-CoV-2-infected dying cells impairs macrophage anti-inflammatory functions and clearance of apoptotic cells
Article Snippet: Samples were imaged using a Zeiss LSM 780 laser scanning confocal microscope (Carl Zeiss), postprocessed for brightness and contrast adjustment with ImageJ image processing package (NIH), and layouts were built on Adobe Photoshop. .. For the engulfment assays by flow cytometry, macrophages were labeled with 5 μM CellTrace Violet dye (CTV, Thermo Scientific), according to the manufacturer’s recommendations. .. CellTrace Violet (CTV)-labeled macrophages were stimulated with UV-AC or CoV2-AC previously stained with 1 μM CellTrace Far Red dye (CTFR, Thermo Scientific) or 5 μM CellTrace CFSE dye, as described in the legend of the figures.

Irradiation:

Article Title: TIGIT regulates apoptosis of risky memory T cell subsets implicated in belatacept-resistant rejection.
Article Snippet: .. Irradiated stimulator PBMCs (8 × 105) were labeled with CellTrace Violet dye (CTV, ThermoFisher) with 1 μl of 5 mM CTV per 107 cells at RT for 20 minutes, and then cultured with responder PBMCs (4 × 105) in culture medium R10 as described above with 10% plasma from the responders for 6 days at 37°C in a 5% CO2, humidified atmosphere. ..

Labeling:

Article Title: Microphysiological Drug‐Testing Platform for Identifying Responses to Prodrug Treatment in Primary Leukemia
Article Snippet: .. To simplify the FACS analysis at the end of the experiment, PDX cells were labeled with CellTrace Violet dye (CTV; C34557, Thermo Fisher Scientific) according to the manufacturer's protocol. ..

Cell Culture:

Clinical Proteomics:

FACS:

Incubation:

Article Title: Loss of NEDD8 in cancer cells causes vulnerability to immune checkpoint blockade in triple-negative breast cancer.
Article Snippet: .. On the next day, healthy donorderived primary human lymphocytes were incubated in PBS containing 1.42 nM CellTrace Violet dye (CTV, Thermo Fisher Scientific) and incubated in the dark for 10min. ..

Article Title: Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis
Article Snippet: 1.5μM, Merck, cat: SRC508) in serum-free complete RPMI (supplemented with 1× GlutaMAX, 1mM Sodium pyruvate, 10mM HEPES, 50 U/ml Penicillin/Streptomycin and 50μM 2-Mercaptoethanol, pH = 7-7.4) for 45 mins at 37°C and 5% CO2. .. CellTrace Violet dye (CTV, Thermo Fisher Scientific) at 10μM was added directly at the 30th min of TAT-Cre incubation and cells were incubated for an additional 15 min at 37°C with CTV and TAT-Cre. ..

Article Title: Loss of NEDD8 in cancer cells causes vulnerability to immune checkpoint blockade in triple-negative breast cancer
Article Snippet: .. On the next day, healthy donor-derived primary human lymphocytes were incubated in PBS containing 1.42 nM CellTrace Violet dye (CTV, Thermo Fisher Scientific) and incubated in the dark for 10 min. After washing twice with PBS, lymphocytes (3 million cells/ml) were added to the tumor-loaded plate in 100 μl culture medium. .. FDA-approved checkpoint inhibitors, nivolumab (Bristol-Myers Squibb) or durvalumab (AstraZeneca) were added to the TICS plate at a final concentration of 10 μg/ml in order to inhibit the PD-1/L1 pathway.

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